Cryo-EM Sample Preparation

The Cryo-EM sample preparation process has multiple workflows, each with their own intricacies and potential problem points. These tools are designed to improve the efficiency of process, resulting in a higher yield of high quality samples, and to make steps of the workflows more ergonomic, and your life generally easier.

Grid Boxes designed to eradicate the long felt drawbacks of conventional grid boxes that hold either 4 unclipped grids or 4 autogrids.

Tools that improve the efficiency of the Vitrification workflow with the Vitrobot, by providing higher quality grids and by making the manual steps easier.

Products that enable the clipping of grids and their manipulation and ensure the quality of the clipping.

Items that are useful for the general workflows of cryoEM sample preparation.

Repair services for tools used in the cryoEM sample preparation workflows, providing "like-new" tools for a significantly cheaper price and in a sustainable fashion. Furthermore, NanoSoft offers materials characterization services with cryoEM.

Grid Boxes

 

Hybrid Grid Box

Bulk Storage Grid Box

Hybrid Grid Box

Hybrid Grid Box's patented design has separate pockets for unclipped grids and autogrids, securely cradling samples while allowing for unmatched ease in placement and removal. Makes for easier workflow with fewer grid boxes needed and easier traceability of samples from vitrification through imaging.

Bulk Box with 12 Autogrids

The Bulk Storage Grid Box's patented design stores 12 autogrids optimally, and can also hold 12 unclipped grids comfortably in the same slots. Storage for an entire cassette of grids from vitrification through to data acquisition. Optimal storage density and sample traceability in one grid box.

Vitrification

 

Cryo-Tweezers

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The Cryo-Tweezers have modified reverse-action tweezers so that you don't have to fight with the Vitrobot tweezers and the black clip to hold grids.  Plus the spring-loaded clamp makes placing and removing the tweezers from the plunge arm much smoother, making your life easier and reducing the number of wasted grids.

Igloo

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The Igloo interfaces with the Vitrobot vitrification dewar to reduce cryoEM sample contamination through multiple features, and creates an overall easier sample preparation process, leading to improved sample preparation efficiency. 

Vitrobot Foam Dewar & Metal Parts

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The foam Vitrification Dewar and Metal Parts are compatible with the Vitrobot, and all of the parts are interchangeable with FEI versions of the foam dewar and metal parts. Furthermore, the dewar design provides an improved interface with the Igloo, creating a better seal to contain cold nitrogen gas.

Ethane Condenser

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Easy to place condenser cap interfaces securely with the Vitrobot spider enabling "one-handed" ethane condensing, where the user can focus solely on controlling the ethane flow rate and the level of liquid ethane.

Quick Lid

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Easy to place lid can be quickly placed onto grid boxes during vitrification sessions to protect already frozen grids while they wait for the session to be complete. The weight of the stainless steel part keeps it securely in place.

Clipping

Autogrid Tweezers

Autogrid Inspection Tool

Reverse Action Autogrid Tweezers

 
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Autogrid tweezers for manipulation of autogrid cartridges, with same dimensions as the Thermo Fisher version, but with a significantly lower price.

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A reverse-action version of our Autogrid Tweezers, with the same notched tips but with the convenience and security of reverse-action tweezers.

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Tool enables a final check of clipping quality before loading autogrids into the autoloader cassette, potentially saving weeks in TEM down time, which is likely in the case of loading a poorly clipped grid that comes unclipped in the autoloader or column.

General Sample Preparation 

 

Spearlab Tall Dewar

Spearlab Standard Vessel

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Spearlab Tall Dewar is a foam vessel capable of holding LN2 and is perfectly sized to slip cryoEM puck canes into to place into/remove from transport/storage dewars at cryo temperature during sample retrieval

Spearlab Standard Vessel is a foam container capable of holding LN2 and the perfect size for any grid box or grid workflow steps/processing necessary

Services

 

Clipping Tool Repair

Cryo-Tweezer/Vitrobot Tweezer Repair

Cryo-TEM Materials Characterization

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The inside barrel of the clipping tool will wear over time. This cumulative damage often results in difficulty seating C-clips at the tip of the tool without them popping out prematurely. Our service makes the barrel like new.

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Tweezer tips are delicate and can easily be damaged with normal laboratory use.  Rather than discard a tweezer with damaged tips, we can repair them and send them back to you in like-new condition.

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We utilize nanomaterials expertise and state-of-the-art instrumentation to characterize nanomaterials, including polymer nanoparticles, liposomes, vesicles, micelles and any type of particle architecture.